What is an ANA (Antinuclear Antibody) with HEp-2 substrate Test?

Antibodies are proteins that are made by the immune system of the body to fight foreign substances, like bacteria and viruses. ANAs are classified into two groups, antibodies to DNA and histones and antibodies to nuclear material. The antinuclear antibody attacks one's cells by mistake and is called an autoantibody. An ANA IFA test is a blood test that looks for antinuclear antibodies in the blood. They are referred to as antinuclear tests because they target the nucleus of the cell. This test detects antibodies that bind to an antigen mixture adhering to a solid surface which replaces the HEp-2 cells.

When does the doctor prescribe ANA (antinuclear antibody) IFA with reflex titre with HEp-2 substrate test?
A doctor may prescribe this test when he suspects

Scleroderma
Rheumatoid arthritis
Addison disease
Sjogren's syndrome
Autoimmune Hepatitis

What are the components of the (antinuclear antibody) ANA with HEp-2 substrate test?

There are three primary methods for screening ANA IFA hep 2 tests:

IFA detects antibodies that attach to a tissue substrate, which, for ANAs, is often fixed HEp-2 cells.
Enzyme immunoassay (EIA) detects antibodies that bind to an antigen mixture adhering to a solid surface which replaces the HEp-2 cells.
Polystyrene bead sets used in multiplex immunoassays (MIA) are identified from one another by their fluorescent signature.

What is the normal range of the ANA with HEp-2 substrate test?

A negative report indicates there is no antinuclear antibodies in the blood,
A positive report indicates that antinuclear antibodies were detected in the blood, and one might have an autoimmune disease. However, if the report is positive, the doctor may prescribe more tests to make a final diagnosis.

Note: Sometimes, healthy people also have antinuclear antibodies in their blood. Therefore having antibodies in the blood doesn't always mean that the patient has an autoimmune disorder. One has to consult their treating physician. Knowing the ANA titer and pattern can help interpret positive ANA results

What are the prerequisites for the antinuclear antibody with HEp-2 substrate test?

The sample is collected from the vein
No special preparations are required for an ANA test.
There is no risk to the patient.
Results from an antinuclear antibody test alone cannot diagnose a specific disease.

What is an ANA (Antinuclear Antibody) with HEp-2 substrate Test?

​When does the doctor prescribe ANA (antinuclear antibody) IFA with reflex titre with HEp-2 substrate test?A doctor may prescribe this test when he suspects

What are the components of the (antinuclear antibody) ANA with HEp-2 substrate test?

There are three primary methods for screening ANA IFA hep 2 tests:

What is the normal range of the ANA with HEp-2 substrate test?

What are the prerequisites for the antinuclear antibody with HEp-2 substrate test?

When does the doctor prescribe ANA (antinuclear antibody) IFA with reflex titre with HEp-2 substrate test?
A doctor may prescribe this test when he suspects

Anti-Nuclear Antibody / Factor (ANA / ANF) - IFA (HEP-2) in Dilutions Test in Kottayam

A0270

Monday - Saturday

No Special Preparation Required

24 Hours

₹2,900

Parameters

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