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There are different types of hemoglobin present in human blood. The hemoglobin HPLC test is used to detect the level of various types of hemoglobin in human blood. Based on the test results, doctors can find the presence of abnormal hemoglobin variants, which can be used to diagnose serious medical conditions like thalassemia and sickle cell anaemia. The test can be used alongside other diagnostic tests to confirm the presence of the conditions mentioned above. The hemoglobin HPLC test is used in response to symptoms presented and is often recommended to patients of all ages.
The HB electrophoresis or hemoglobin HPLC test is prescribed by doctors in response to medical symptoms presented by patients. This test can also be used as a screening test to check for the risk of thalassemia in patients with higher risk factors. Here are some of the common symptoms that warrant doctors to prescribe a HPLC for hemoglobin test.
Symptoms of anaemia such as weakness and paleness of skin
Enlarged spleen that’s examined medically
Delayed growth in children
Dark urine colour despite adequate hydration
Based on the reports of the hemoglobin HPLC test, doctors can start treatment processes for conditions like thalassemia. The results of screening tests can be used by doctors to prescribe or recommend preventative measures to minimise the risk of thalassemia in patients.
The HPLC for hemoglobin test has various testing parameters, including hemoglobin Hb A, Hb A2, Hb A2/E, Hb C, Hb D, Hb E, Hb F, and Hb S. The HB electrophoresis test results are expressed as positive or negative based on the presence of certain hemoglobin types in the sample. The testing methodology for HB electrophoresis and hemoglobin HPLC tests are different. At Aster, we ensure the sample is tested using both methods to ensure accurate test results. High levels of HbA2 or HbF hemoglobin in the sample are indicative of thalassemia. Similarly, the presence of HbS in the sample can indicate sickle cell anaemia.
The hemoglobin HPLC test is performed by drawing blood from the veins of the patient’s arm and sending it for testing. There are no specific prerequisites for undergoing this test. However, patients are required to mention the date of birth, sex, race, and history of blood transfusion on the test request form.